The UNC Charlotte Microarray Core Facility is a joint project by UNCC and
Carolinas Medical Center researchers and primarily funded by NC
Biotech. The facility is equipped for small-batch printing of
oligonucleotide and cDNA glass-slide microarrays. The facility grant
was awarded in August of 2006. The facility, located in 273 CARC, is
supervised by Dr. Cynthia Gibas and staffed by a full-time technician,
and will be accessible to all UNCC researchers on a cost-recovery basis
beginning in spring 2007.
Major equipment:
- Matrix
PlateMate 2x2 robotic system for automated microvolume pipetting,
including plate replication and reformatting, reagent addition, serial
dilution, and reaction setup;
- BioRad
BioOdyssey Calligrapher benchtop microarray fabrication system for
printing of small- to medium- batches of microarrays on standard
slides, on membranes or in microplates;
- Tecan HS-4800 automated hybridization and wash station for processing of multiple microarrays under controlled conditions;
- Tecan
LS-Reloaded microarray scanner with capabilities for multi-slide and
microplate scanning and up to three dye chemistries simultaneously in
red, green and blue wavelength ranges;
Other equipment in the core facility includes a Bio-Rad iQ5 Real Time
PCR system with 5-color detection capabilities, an Agilent eBioanalyzer
for QC of nucleic acid and protein samples, a NanoDrop UV-Vis
spectrophotometer for quantitation of nucleic acids in extremely small
sample volumes, and ThermoElectron and Agilent hybridization ovens,
carousels, and chambers for manual hybridization of glass-slide
microarrays.
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Staff: Mr. Joshua Newton, 273A Cameron Applied Research Center
